Using GFP as a Scorable Marker in Walnut Somatic Embryo Transformation
نویسندگان
چکیده
منابع مشابه
agrobacterium mediated transformation of somatic embryos of persian walnut using fld gene for osmotic stress tolerance
somatic embryos of persian walnut were transformed with two strains of agrobacterium tumefaciens i.e. lba4404 and c58, and two plasmids, namely, pbi121 with nptii and gus genes for improving the transformation protocol, and p6u-ubi-fvti plasmid containing the hpt and fld genes. the transformation frequency was 10%. pcr and rt-pcr analysis proved the presence and expression of the genes. the tra...
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ژورنال
عنوان ژورنال: Annals of Botany
سال: 2000
ISSN: 0305-7364
DOI: 10.1006/anbo.2000.1143